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Proposal document
Astrocyte calcium signaling in sleep-stage transitions
NIH R21 · draft 4 · Kestrel Lab (illustrative)
Specific Aims

We propose that astrocyte calcium dynamics gate sleep-stage transitions, tested with two-photon imaging across 240 sessions in aim 2, extending the aims narrative to a second page.

Aim 1 builds the pipeline with the Marsh group; this resubmission is filed 14 months after the original application.

Letters of support

Dr. A. Okafor (Marsh group) confirms the collaboration on aim 1 and access to the shared imaging core.

AttachmentsAims.pdfBiosketches.pdfBudget justification — missing
Rule checks3 open · 1 resolved
Page limit
Specific Aims may not exceed one page — draft 4 runs 1.2
“The Specific Aims attachment is limited to one page.”
nih-forms-h/research-plan/aims/req-2
Required statement
Okafor letter names the role but not the specific contribution
“Letters of support must stipulate the specific contribution of the collaborator to the project.”
nih-gps/2.5.1/letters/req-11
Missing document
Budget justification required for a detailed R&R budget
nih-forms-h/budget/justification/req-1
Eligibility window✓ Resolved
Resubmission filed within the 37-month window — 14 months
nih-gps/2.3.7/resubmission/req-4

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52 more NIH activity codes are in the catalog; their workspaces follow by application volume.

R01, newNIH Research Project Grant (R01) · PA-25-30123 documents · 1,631 rules · Next due Feb 5, 2027 — 115 days
Which R01?
Specific Aims4 rules2 open1 page — the goals, expected outcomes and impact of the proposed research, and its specific objectives
Research Strategy19 rulesChecked12 pages — Significance, Innovation, Approach
Project Summary/Abstract18 rulesUploaded30 lines — the broad objectives and specific aims, for a general audience
Project Narrative2 rulesDraft2–3 sentences — relevance to public health
Bibliography & References Cited6 rulesUploadedNo page limit — every publication cited in the application, with the PMC ID where the public access policy applies
Facilities & Other Resources10 rulesNot startedNo page limit — how the scientific environment contributes to the probability of success
Equipment3 rulesNot startedNo page limit — major items already available for the project
Biographical Sketches8 rulesDraftNo hard page limit — the Common Form and NIH supplement, whose length SciENcv sets, for every senior/key person and other significant contributor
Budget workbook106 rulesSoonComputes the R&R budget by period from your institution's rates and lab roster, or the modular budget when direct costs are $250,000 or less a year
Budget Justification74 rulesCheckedNarrative bound to the R&R budget lines; for a modular budget, the personnel, consortium and additional-narrative justifications
Consortium/Contractual Arrangements5 rulesUploadedThe programmatic, fiscal and administrative arrangements, with an R&R subaward budget per site
Multiple PD/PI Leadership Plan7 rulesDraftGovernance, roles, communication and conflict resolution
Letters of Support12 rulesUploadedConsultants, collaborators and institutional commitments, appended as one PDF
Vertebrate Animals13 rulesNot startedDescription, justification, minimization of pain and distress, euthanasia
Human Subjects & Clinical Trials Information69 rulesNot startedThe PHS form with a study record per study, or a delayed-onset justification
Resource Sharing PlanDraftHow model organisms and research tools produced by the project will be shared
Authentication of Key Biological and/or Chemical Resources3 rules2 open1 page — how key resources will be authenticated
Data Management & Sharing Plan44 rulesCheckedRecommended 2 pages — the elements of the NIH policy, with its costs on the budget
Appendix19 rulesUploadedAllowable materials only — blank data-collection instruments, surveys and interview protocols; no publications
Cover Letter18 rulesDraftOptional — notes to NIH staff with the SF 424 (R&R); assignment preferences go on the PHS Assignment Request Form, never here
PHS Assignment Request Form6 rulesUploadedOptional — institute, study section and reviewer preferences, on the form NIH provides for them
Other Attachments5 rulesNot startedSelect Agent Research, a foreign-component justification, and anything the announcement calls for
Submission & general88 rulesNot startedSF 424 (R&R), PHS 398 Cover Page Supplement, Project/Performance Sites — registrations, formats, page limits and due dates
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